Composite

Part:BBa_K4164010

Designed by: Bo Cheng   Group: iGEM22_NAU-CHINA   (2022-09-30)


constitutive expression of RXR-ddRFPB1

In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we tried low intensity promoter at first.This composite part contains the low intensity promoter (BBa_J23105) and RBS. The composite part can be directly imported into plasmid and express RXR-ddRFPB1. If you want to learn about the detailed introduction of RXR-ddRFPB1, you can click the link below. https://parts.igem.org/Part:BBa_K4164997

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1928
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 418
    Illegal AgeI site found at 1445
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 800


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Parameters
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